Canine monocytic ehrlichiosis in Entre Ríos province, Argentina: molecular confirmation of cases in Concordia city
DOI:
https://doi.org/10.14409/favecv.v19i1.8935Keywords:
Ehrlichia canis, Canine Granulocytic Ehrlichiosis, Rhiphicephalus sanguineus, Trombocytopenia, ArgentinaAbstract
The aim of this work was to confirm the presence of Ehrlichia canis in blood samples of dogs from Concordia, Entre Ríos Province, Argentina. DNA was extracted in 14 blood samples of dogs with presumptive diagnosis of CME. A PCR protocol targeting dsb gene, specific for genus Ehrlichia was carried out for detection and species confirmation was carried out by sequencing. Twelve out of 14 samples shown bands of the expected size. The obtained sequences revealed 98-100% identity with E. canis sequences registered in GenBank. Moreover, ticks were retrieved from an E. canis positive dog and a fragment of mitochondrial ARNr 16S gene was amplified. The obtained sequences were identified as R. sanguineus sensu stricto (temperate linage). This work confirmed the presence of E. canis in Concordia city, Entre Ríos province resulting in an alert for veterinary clinicians in this region aiming to encourage prevention strategies of this disease and vector control.
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