Comparison of 3 real time RT-PCR kits for SARS-CoV-2 detection

Authors

DOI:

https://doi.org/10.14409/favecv.v20iSuppl..10131

Keywords:

Molecular diagnosis, GeneFinder, BGI, DisCoVery, SARS-CoV-2

Abstract

The objective of the present study was to evaluate the diagnostic performance of 3 commercial SARS-CoV-2 real time RT-PCR detection kits on nasopharyngeal swab samples, using the majority criterion. One hundred RNA samples informed as detectable (n=50) and not detectable (n=50) with GeneFinder-COVID-19 Plus RealAmp were selected, and later analyzed with BGI-Real-Time Fluorescent for Detecting SARS-CoV-2 and DisCoVery-SARS-CoV-2 RT-PCR. Agreement between tests was almost perfect; sensitivity was 100% for Genefinder and DisCoVery and less than 90% for BGI. Real time PCR products from 10 discordant samples were further analyzed by agarose gel electrophoresis, confirming 3 false negatives by BGI and suggesting 5 samples as true positives by DisCoVery. The use of DisCoVery and GeneFinder™ appear as an excellent choice with good sensitivity and specificity values.

References

Arevalo-Rodriguez I, Buitrago-Garcia D, Simancas-Racines D, Zambrano-Achig P, del Campo R, Ciapponi A, Sued O, Martinez-Garcia L, Rutjes A, Low N, Perez-Molina JA, Zamora J. 2020. False-negative results of initial RT-PCR assays for covid-19: a systematic review. PLoS One 15: e0242958.

Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DKW, Bleicker T, Brünink S, Schneider J, Schmidt ML, Mulders DGJC, Haagmans BL, van der Veer B, van den Brink S, Wijsman L, Goderski G, Romette JL, Ellis J, Zambon M, Peiris M, Goossens H, Reusken C, Koopmans MPG, Drosten C. 2020. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 25: 2000045.

Lu R, Zhao X, Li J, Niu P, Yang B, Wu H, Wang W, Song H, Huang B, Zhu N, Bi Y, Ma X, Zhan F, Wang L, Hu T, Zhou H, Hu Z, Zhou W, Zhao L, Chen J, Meng Y, Wang J, Lin Y, Yuan J, Xie Z, Ma J, Liu WJ, Wang D, Xu W, Holmes EC, Gao GF, Wu G, Chen W, Shi W, Tan W. 2020. Genomic characterization and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet 395: 565-74.

Moore NM, Li H, Schejbal D, Lindsley J, Hayden MK. 2020. Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2. J. Clin. Microbiol. 58: e00938-20.

Moré G, Panei CJ, Fuentealba N, Aspitia C, Bravi ME, Origlia J, Rambeaud M, Tizzano MA, Rudd Garcés G, Golijow C, Unzaga JM, Pecoraro M. 2020. Resultados del primer bimestre de trabajo de la unidad de diagnóstico COVID-19 de la Facultad de Ciencias Veterinarias-UNLP. Analecta Veterinaria 40: https://doi.org/10.24215/15142590e050

Ramirez JD, Muñoz M, Hernandez C, Flórez C, Gomez S, Rico A, Pardo L, Barros EC, Paniz-Mondolfi AE. 2020. Genetic Diversity Among SARS-CoV2 Strains in South America may Impact Performance of Molecular Detection. Pathogens 9: 580.

Shi J, Han D, Zhang R, Li J, Zhang R. 2020. Molecular and serological assays for SARS-CoV-2: insights from genome and clinical characteristics. Clin. Chem. 66: 1030-46.

Watson J, Whiting PF, Brush JE. 2020. Interpreting a covid-19 test result. Brit. Med. J. 369: m1808.

Woloshin S, Patel N, Kesselheim AS. 2020. False Negative Tests for SARS-CoV-2 Infection — Challenges and Implications. New Engl. J. Med. 383: e38.

Ye ZW, Yuan S, Yuen KS, Fung SY, Chan CP, Jin DY. 2020. Zoonotic origins of human coronaviruses. Int. J. Biol. Sci. 16: 1686-97.

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Published

2021-07-07

How to Cite

MORÉ, G., TIZZANO, M. A., RAMBEAUD, M., PANEI, C. J., FUENTEALBA, N., ASPITIA, C., BRAVI, M. E., ORIGLIA, J., RUDD GARCÉS, G., GOLIJOW, C., UNZAGA, J. M., & PECORARO, M. (2021). Comparison of 3 real time RT-PCR kits for SARS-CoV-2 detection. FAVE Sección Ciencias Veterinarias, 20(Suppl.), 3–8. https://doi.org/10.14409/favecv.v20iSuppl.10131

Issue

Vol. 20 No. Suppl. (2021): FAVE Sección Ciencias Veterinarias - Supplement

Section

Short communications

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