Ability of Lactiplantibacillus plantarum LP5 to inhibit biofilms of Campylobacter coli
DOI:
https://doi.org/10.14409/favecv.2022.1.e0002Keywords:
biofilms, abiotic surface, Campylobacter coli, Lactiplantibacillus plantarumAbstract
The objective of this study was to evaluate the inhibitory capacity of Lactiplantibacillus plantarum LP5 against Campylobacter coli in in vitro biofilm formation and competitive exclusion assays. Biofilm formation by C. coli NCTC11366, C. coli DSPV458, C. coli DSPV541 and C. coli DSPV570 was evaluated by OD measurement. The inhibitory capacity of L. plantarum LP5 against C. coli was evaluated on glass, nylon and aluminium discs, added with L. plantarum and incubated at 37°C for 72 h. C. coli was added to each washed well. The plates were incubated at 42°C for 72 h in microaerophilic conditions and the biofilms were detached for quantification. The four strains of C. coli were classified as moderate biofilm former. The competitive exclusion test showed that the biofilm formation of the C. coli strains in all materials was significantly higher than the biofilm formation of each pathogen in the presence of L. plantarum LP5 biofilms. Although it is necessary to carry out more tests to confirm the ability of C. coli to survive in hostile environments until reaching the host, this study would allow progress in the elucidation of its behaviour through the formation of biofilms.
References
Cáceres ME, Etcheverría AI, Padola NL. 2019. Efectos del medio de cultivo y de la metodología aplicada sobre la formación de biopelículas de 2 cepas de Escherichia coli diarreagénicas. Rev. Argent. Microbiol. 51: 208-213.
Camargo AC, Woodward JJ, Call DR, Nero LA. 2017. Listeria monocytogenes in food-processing facilities, food contamination, and human listeriosis: the Brazilian scenario. Foodborne Pathog. Dis. 14: 623-636.
Castellano P, Belfiore C, Fadda S, Vignolo G. 2008. A review of bacteriocinogenic lactic acid bacteria used as bioprotective cultures in fresh meat produced in Argentina. Meat. Sci. 79: 483-499.
Gómez NC, Ramiro JM, Quecan BX, de Melo Franco BD. 2016. Use of potential probiotic lactic acid bacteria (LAB) biofilms for the control of Listeria monocytogenes, Salmonella Typhimurium, and Escherichia coli O157: H7 biofilms formation. Front. Microbiol. 7: 863.
Guerrieri E, de Niederhäusern S, Messi P, Sabia C, Iseppi R, Anacarso I, Bondi M. 2009. Use of lactic acid bacteria (LAB) biofilms for the control of Listeria monocytogenes in a small-scale model. Food Control. 20: 861-865.
Gunther IV NW, Chen CY. 2009. The biofilm forming potential of bacterial species in the genus Campylobacter. Food Microbiol. 26: 44-51.
Kalmokoff M, Lanthier P, Tremblay TL, Foss M, Lau PC, Sanders G, Szymanski CM. 2006. Proteomic analysis of Campylobacter jejuni 11168 biofilms reveals a role for the motility complex in biofilm formation. J. Bacteriol. 188: 4312-4320.
Monteiro GP, Rossi DA, Valadares EC, Peres PAB, Braz RF, Notário FO, Fonseca BB. 2021. Lactic bacterium and Bacillus sp. biofilms can decrease the viability of Salmonella gallinarum, Salmonella heidelberg, Campylobacter jejuni and methicillin resistant Staphylococcus aureus on different substrates. Rev. Bras. Cienc. Avic. 23: 1-12.
Morales P, Aguirre J, Troncoso M, Figueroa G. 2020. Comparison of in vitro and in situ antagonism assays as tools for the selection of bio-preservative lactic acid bacteria (LAB) in poultry meat. LWT. 118: 108846.
Reeser RJ, Medler RT, Billington SJ, Jost BH, Joens LA. 2007. Characterization of Campylobacter jejuni biofilms under defined growth conditions. Appl. Environ. Microbiol. 73: 1908-1913.
Rossler E, Fuhr EM, Lorenzón G, Romero-Scharpen A, Berisvil AP, Blajman JE, Astesana DM, Zimmermann JA, Fusari ML, Signorini ML, Soto LP, Frizzo LS, Zbrun MV. 2017. Presencia de serotipos de Campylobacter jejuni O:19 en la cadena avícola Argentina. Rev. Argent. Microbiol. 49: 178-182.
Ruiz MJ. 2019. Aislamiento e identificación de bacterias ácido lácticas con actividad inhibitoria de bacterias implicadas en enfermedades transmitidas por alimentos. Tesis doctoral, FCV-UNICEN. 124 pp.
Ruiz MJ, Zbrun MV, Signorini ML, Zimmermann JA, Soto LP, Rosmini MR, Frizzo LS. 2021. In vitro screening and in vivo colonization pilot model of Lactobacillus plantarum LP5 and Campylobacter coli DSPV 458 in mice. Arch. Microbiol. 1-11.
Shokri D, Khorasgani MR, Mohkam M, Fatemi SM, Ghasemi Y, Taheri-Kafrani A. 2018. The inhibition effect of lactobacilli against growth and biofilm formation of Pseudomonas aeruginosa. Probiotics Antimicrob. Proteins. 10: 34-42.
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