Studies on the efficacy of pyrantel for the control of small strongyles in equines in central Argentina

Authors

  • Laura Gabriela Cooper Universidad Católica de Córdoba
  • Benjamín José Paz Benard Universidad Católica de Córdoba
  • Gabriel Caffe Universidad Católica de Córdoba - Universidad Nacional de Villa María
  • Ignacio Fernández Llanos Universidad Católica de Córdoba
  • Franco José Arroyo Universidad Católica de Córdoba
  • Oscar Sergio Anziani Universidad Católica de Córdoba

DOI:

https://doi.org/10.14409/favecv.2023.22.e0024

Keywords:

horses, cyathostomins, pyrantel, resistance, Argentina

Abstract

The objective of this study was to investigate the prevalence of resistance to pyrantel pamoate in 11 horse farms (111 horses) from the central area of ​​Argentina using the Fecal Egg Count Reduction Test (FECRT) and a hierarchical Bayesian model for data analysis. The same methodology was applied on week 4 post treatment to detect possible shortening in the ERP. The general efficacy of pyrantel was considered adequate in 9/11 farms, resistant in one and suspicious on other, and no changes were observed in the ERP. In the elimination of eggs before treatment (n= 127), 23.7 % of the animals were considered low eliminators, 32.5 % as moderate eliminators and 43.8 % as high eliminators, emphasizing the possibility of selectively treating the latter. Selective treatments would allow preserving the useful life of this drug, as well as that of the macrocyclic lactones, and represent a more rational alternative (compared to current mass treatments) for the sustainable control of small strongyles in adult horses. In the pre-treatment stool cultures, small strongyles were the parasitic group mostly observed but the presence of Strongylus vulgaris was discovered in three of the 11 farms studied, which warrants future research to determine the prevalence of this parasite of recognized pathogenicity. Key words: horses, cyathostomins, pyrantel, resistance, Argentina.

References

Anziani OS, Catanzaritti H. 2005. Resistencia a los benzimidazoles en nematodes de los equinos en Santa Fe, Argentina. Vet. Arg. 218: 571-578.

Anziani OS, Ardusso G 2017. Resistencia a los antihelmínticos en nematodes intestinales que parasitan a los equinos en la Argentina. INTA-RIA. 43: 24-35.

Anziani OS, Muchiut S, Cooper L, Cerutti J. 2016. Small strongyles (cyathostomes) and benzimidazoles. Persistance of status of resistance after nine years without the use of these drugs and efficacy of ivermectin about this parasite population. J. Equine Vet. Sci. 39: 52-55. DOI: 10.1016/j.jevs.2016.02.115

Becher AM, Mahling M, Nielsen MK, Pfister K. 2010. Selective anthelmintic therapy of horses in the Federal states of Bavaria (Germany) and Salzburg (Austria): an investigation into strongyle egg shedding consistency. Vet. Parasitol. 171:116-122. DOI: 10.1016/j.vetpar.2010.03.001

Boersema JH, Borgsteede FH, Eysker M, Saedt I. 1995. The reappearance of strongyle eggs in faeces of horses treated with pyrantel embonate. Vet. Q. 17: 18-20. DOI: 10.1080/01652176.1995.9694524

Boersema JH, Eysker M, Maas J, van Der Aar WM. 1996. Comparison of the reappearance of strongyle eggs on foals, yearlings and adult horses after treatment with ivermectin or pyrantel. Vet. Q. 18: 7-9. DOI: 10.1080/01652176.1996.9694602

Brady H & Nichols W. 2009. Drug resistance in equine parasites: an emerging global problem. J. Equine Vet. Sci. 29: 285-295. https://doi.org/10.1016/j.jevs.2009.04.186

Carroll CL & Huntington PJ. 1988. Body condition scoring and weight estimation of horses. Equine Vet. J. 20:41-45. DOI: 10.1111/j.2042-3306.1988.tb01451.x

Cerutti J, Cooper L, Caffe G, Cervilla N, Muchiut S, Anziani OS. 2012. Resistencia de los pequeños estrongilidos (grupo Cyathostomas) a los benzimidazoles en equinos del área central de la Argentina. Rev. InVet. 14: 41-46.

Cooper LG, Paz Benard BJ, Molinelli A, Caffe G, Fernandez Llanos I, Fassola LA, Cerutti J, Anziani OS. 2020. Eliminación de huevos de los pequeños estróngilos en la materia fecal de los equinos y su importancia práctica en la posibilidad de tratamientos selectivos. FAVE Sección Ciencias Veterinarias. 19: 1-6. DOI: 10.14409/favecv.v19i1.8977

Cooper LG, Paz Benard BJ, Nielsen MK, Molineri A, Anziani OS. 2021. A survey of helminth control practices on large horse farms in Argentina. Equine Vet. J. 53: 50-50. DOI:10.1111/evj.71_13495

Cooper LG, Paz Benard BJ, Caffe G, Fernández Llanos I, Anziani OS. 2023. Evaluaciones sobre la eficacia clínica de la ivermectina en el control de los pequeños estróngilos equinos (Cyathostoma spp). Status de susceptibilidad o resistencia en establecimientos del área central de la Argentina. FAVE Sección Ciencias Veterinarias. 22: 1-17. DOI: 0.14409/favecv.2023.22.e0012

Denwood MJ, Kaplan RM, McKendrick IJ, Thamsborg SM, Nielsen MK, Levecke B. 2023. A statistical framework for calculating prospective sample sizes and classifying efficacy results for faecal egg count reduction tests in ruminants, horses and swine. Vet. Parasitol. 314:1-12. DOI: 10.1016/j.vetpar.2022.109867

Döpfer D, Kerssens CM, Meijer YG, Boersema JH, Eysker M. 2004. Shedding consistency of strongyle-type eggs in Dutch boarding horses. Vet. Parasitol. 124: 249-258. DOI: 10.1016/j.vetpar.2004.06.028

Drudge JH, Lyons ET, Tolliver SC, Kubis JE. 1982. Pyrantel in horses: clinical trials with emphasis on a paste formulation and activity on benzimidazole-resistant small strongyles. Vet. Med. Small. Anim. CLIn. 77: 957-967.

Geurden T, De Keersmaecker F, De Keersmaecker S, Claerebout E, Leathwick DM, Nielsen MK, Sauermann CW. 2021. Three-year study to evaluate an anthelmintic treatment regimen with reduced treatment frequency in horses on two study sites in Belgium. Vet. Parasitol. 298:1-16. DOI: 10.1016/j.vetpar.2021.109538

Gokbulut C, McKellar QA. 2018. Anthelmintic drugs used in equine species. Vet. Parasitol. 261: 27-52. DOI: 10.1016/j.vetpar.2018.08.002

Kaplan RM, Denwood MJ, Nielsen MK, Thamsborg SM, Torgerson PR, Gilleard JS, Dobson RJ, Vercruysse J, Levecke B. 2023. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) guideline for diagnosing anthelmintic resistance using the faecal egg count reduction test in ruminants, horses and swine. Vet. Parasitol. 318:1-20. DOI: 10.1016/j.vetpar.2023.109936

Lichtenfels JR, Kharchenko VA, Dvojnos GM. 2008. Illustrated identification keys to strongylid parasites (strongylidae: Nematoda) of horses, zebras and asses (Equidae). Vet.Parasitol. 156:1-158. DOI: 10.1016/j.vetpar.2008.04.026

Losinno SJ, Aguilar J, Carbonetti L, Ferniot E, San Esteban F, Flores Suares CM. A survey on parasite control in sport horses of Argentina and other regional countries. 2018. Vet. Parasitol. Reg. Stud. Reports. 13:74-78. DOI: 10.1016/j.vprsr.2018.04.004

Lyons ET, Tolliver SC, Drudge JH, Stamper S, Swerczek TW, Granstrom DE. 1996. Critical test evaluation (1977-1992) of drug efficacy against endoparasites featuring benzimidazole-resistant small strongyles (population S) in Shetland ponies. Vet Parasitol. 66: 67-73. DOI: 10.1016/s0304-4017(96)00997-1

Macdonald SL, Abbas G, Ghafar A, Gauci CG, Bauquier J, El-Hage C, Tennent-Brown B, Wilkes EJA, Beasley A, Jacobson C, Cudmore L, Carrigan P, Hurley J, Beveridge I, Hughes KJ, Nielsen MK, Jabbar A. 2023. Egg reappearance periods of anthelmintics against equine cyathostomins: The state of play revisited. Int. J. Parasitol. Drugs Drug Resist. 21:28-39. DOI: 10.1016/j.ijpddr.2022.12.002

Nielsen MK, Haaning N, Olsen SN. 2006. Strongyle egg shedding consistency in horses on farms using selective therapy in Denmark. Vet. Parasitol. 135: 333-335. DOI: 10.1016/j.vetpar.2005.09.010

Nielsen MK. 2012. Sustainable equine parasite control: perspectives and research needs. Vet. Parasitol. 185: 32-44. DOI: 10.1016/j.vetpar.2011.10.012

Nielsen MK, Vidyashankar AN, Hanlon BM, Diao G, Petersen SL, Kaplan RM. 2013. Hierarchical model for evaluating pyrantel efficacy against strongyle parasites in horses. 2013. Vet. Parasitol. 197: 614-622. DOI: 10.1016/j.vetpar.2013.04.036

Nielsen MK, Pfister K, von Samson-Himmelstjerna G. 2014. Selective therapy in equine parasite control--application and limitations. Vet Parasitol. 202: 95-103. DOI: 10.1016/j.vetpar.2014.03.020

Nielsen MK. 2015. Universal challenges for parasite control: a perspective from equine parasitology. Trends Parasitol. 31: 282-284. DOI: 10.1016/j.pt.2015.04.013

Nielsen MK, Mittel L, Grice A, Erskine M, Graves E, Vaala W, Tully R, French D, Bowman R, Kaplan R. 2019. AAEP American Association of Equine Practitioners Parasite Control Guidelines. Online at www.aaep.org. Accesso 10 Mayo 2023.

Nielsen MK, von Samson-Himmelstjerna G, Kuzmina TA, van Doorn DCK, Meana A, Rehbein S, Elliott T, Reinemeyer CR. 2022. World Association for the Advancement of Veterinary Parasitology (WAAVP): Third edition of guideline for evaluating the efficacy of equine anthelmintics. Vet. Parasitol. 303:1-9. DOI: 10.1016/j.vetpar.2022.109676

Nielsen MK. 2022. Anthelmintic resistance in equine nematodes: Current status and emerging trends. Int. J. Parasitol. Drugs. Drug. Resist. 20:76-88. DOI: 10.1016/j.ijpddr.2022.10.005

Perdomo R, Cantón G, Fiel C. 2019. Evaluación de la resistencia antihelmíntica en equinos en un establecimiento de la Provincia de Buenos Aires. Universidad Nacional del Centro. 38 pp. Online: https://ridaa.unicen.edu.ar:8443/server/api/core/bitstreams/baaa5137-c345-41fb-98eb-5f74b9d92c23/content. Acceso 10 mayo 2023.

Peregrine AS, Molento MB, Kaplan RM, Nielsen MK. 2014. Anthelmintic resistance in important parasites of horses: does it really matter? Vet. Parasitol. 201: 1-8. DOI: 10.1016/j.vetpar.2014.01.004

Relf VE, Lester HE, Morgan ER, Hodgkinson JE, Matthews JB. 2014. Anthelmintic efficacy on UK Thoroughbred stud farms. Parasitol. Int. 44: 507-514. DOI: 10.1016/j.ijpara.2014.03.006

Russell AF. 1948. The development of helminthiasis in Thoroughbred foals. J. Comp. Path. Therap. 58: 107-127. DOI: 10.1016/s0368-1742(48)80009-3

Scott I, Bishop RM, Pomroy WE. 2015. Anthelmintic resistance in equine helminth parasites –a growing issue for horse owners and veterinarians in New Zealand? N.Z.Vet.J. 63: 188-198. DOI: 10.1080/00480169.2014.987840

Torgerson PR, Paul M, Furrer R. 2014. Evaluating faecal egg count reduction using a specifically designed package "eggCounts" in R and a user friendly web interface. Int. J. Parasitol. 44: 299-303. DOI: 10.1016/j.ijpara.2014.01.005

Vidyashankar AN, Hanlon BM, Kaplan RM. 2012. Statistical and biological considerations in evaluating drug efficacy in equine strongyle parasites using fecal egg count data. Vet. Parasitol. 185: 45-56. DOI: 10.1016/j.vetpar.2011.10.011

von Samson-Himmelstjerna G. 2012. Anthelmintic resistance in equine parasites-detection, potential clinical relevance and implications for control. Vet.Parasitol.185: 2-8. DOI: 10.1016/j.vetpar.2011.10.010

Wang C, Torgerson PR, Kaplan RM, George MM, Furrer R .2018. Modelling anthelmintic resistance by extending eggCounts pack-age to allow individual efcacy. Int. J. Parasit. Drugs Drug Resist. 8:386-393. DOI: 10.1016/j.ijpddr.2018.07.003

Published

2023-10-02

How to Cite

Cooper, L. G., Paz Benard, B. J., Caffe, G., Fernández Llanos, I., Arroyo, F. J., & Anziani, O. S. (2023). Studies on the efficacy of pyrantel for the control of small strongyles in equines in central Argentina. FAVE Sección Ciencias Veterinarias, (22), e0024. https://doi.org/10.14409/favecv.2023.22.e0024