Evaluation of the antagonic capacity of native strains of Trichoderma spp. in front of Verticillium dahliae in the province of Catamarca.

Abstract

The antagonistic capacity of native strains of Trichoderma spp. against Verticillium dahliae, under different culture and incubation conditions. The isolation and molecular identification of antagonistic native strains and the phytopathogen were carried out. They were evaluated in two in vitro tests, using ADP culture medium in two pH conditions and incubated under two lighting conditions. By means of dual culture techniques, a trial was carried out with a completely randomized design with a 2 x 2 x 2 factorial arrangement, and microcultures. The antagonistic capacity of two native strains of Trichoderma, TT (T. hamatum) and M5A (T. hamatum) on the native phytopathogen strain (Verticillium dahliae) was evaluated. The results obtained showed that the TT strain at pH 6.5 with 16 h light / 8 h Darkness had the shortest Contact Time and the highest Growth Inhibition Percentage with 8 h of light /16 h. of darkness. Class 1 was obtained in both tests and treatments, both being efficient in the competition for substrate and space. It was observed that the native strain M5A and the standard strain TT, parasitize the phytopathogen. Therefore, it is concluded that the TT strain has greater antagonistic attributes against the phytopathogen V. dahliae, under conditions of 8 h light / 16 h dark.