Effect of naphthaleneacetic acid, indole-3-butyric acid, and indole-3-acetic acid on the in vitro rhizogenic response of Neltuma alba.
DOI:
https://doi.org/10.14409/fa.2024.23.e0026Keywords:
auxins, Neltuma sp., conservation, adventitious roots, explantsAbstract
Neltuma alba, known as “algarrobo blanco” is an important native species in the Chaco ecoregion, particularly in Argentina, due to its biological, productive, and cultural value. Overexploitation of its timber is causing a loss in the species' genetic diversity. Vegetative propagation in vitro is emerging as a biotechnological tool for conserving forest genetic resources. This study aimed to assess the effects of different types and concentrations of auxins on the in vitro rooting response of N. alba. The results indicate that explants have the ability to develop adventitious roots with or without the presence of plant growth regulators, though higher concentrations of auxins induce a better rooting response. Subculturing explants in an auxin-free medium after 7 days of exposure to 4 mg/l of IBA has shown to reduce callus formation and minimize root malformations. This working methodology allows achieving a 75% survival rate of ex vitro plants.
References
Arce, P., y Balboa, O. (1991). Seasonality in rooting of Prosopis chilensis cuttings and in-vitro micropropagation. Forest Ecology and Management, 40(3-4), 163-173. https://doi.org/10.1016/0378-1127(91)90036-U
Arya, H. C., y Shekhawat, N. S. (1986). Clonal multiplication of tree species in the Thar desert through tissue culture. Forest ecology and management, 16(1-4), 201-208. https://doi.org/10.1016/0378-1127(86)90020-4
Batchelor, C. A., Yao, D., Koehler, M. J., y Harris, P. J. C. (1989). In vitro propagation of Prosopis species (P. chilensis, P. cineraria and P. juliflora). In Annales des Sciences Forestieres (Vol. 46, No. Supplement, pp. 110s-112s). EDP Sciences.
Boeri, P., y Sharry, S. (2018). Somatic embryogenesis of alpataco (Prosopis alpataco L.). Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants: Volume II, 189-198. https://doi.org/10.1007/978-3-319-79087-9_16
Brondani, G. E., de Wit Ondas, H. W., Baccarin, F. J. B., Gonçalves, A. N., y de Almeida, M. (2012). Micropropagation of Eucalyptus benthamii to form a clonal micro-garden. In Vitro Cellular & Developmental Biology-Plant, 48, 478-487. DOI 10.1007/s11627-012-9449-9
Buendía-González, L., Orozco-Villafuerte, J., Cruz-Sosa, F., Chávez-Ávila, V. M., y Vernon-Carter, E. J. (2007). Clonal propagation of mesquite tree (Prosopis laevigata Humb. & Bonpl. ex Willd. MC Johnston). I. Via cotyledonary nodes. In Vitro Cellular & Developmental Biology-Plant, 43, 260-266. DOI 10.1007/s11627-007-9027-8
Buendía-González, L., Estrada-Zúñiga, M. E., Orozco-Villafuerte, J., Cruz-Sosa, F., y Vernon-Carter, E. J. (2012). Somatic embryogenesis of the heavy metal accumulator Prosopis laevigata. Plant Cell, Tissue and Organ Culture, 108, 287-296. DOI 10.1007/s11240-011-0042-4
Caro, L. A., Polci, P. A., Lindström, L. I., Echenique, C. V., y Hernandez, L. F. (2002). Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants. BIOCELL, 26(1), 25-33.
Castillo de Meier, G., y Bovo, O. A. (2000). Plant regeneration from single-nodal-stem explants of legume tree Prosopis alba Griseb. Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopia Electronica, 24(2), 89-95.
De Klerk, G. J., Brugge, J. T., y Marinova, S. (1997). Effectiveness of indoleacetic acid, indolebutyric acid and naphthaleneacetic acid during adventitious root formation in vitro in Malus ‘Jork 9’. Plant cell, tissue and organ culture, 49(1), 39-44.
de Souza, J. C. A. V., Bender, A. G., Tivano, J. C., Barroso, D. G., Mroginski, L. A., Vegetti, A. C., y Felker, P. (2014). Rooting of Prosopis alba mini-cuttings. New Forests, 45, 745-752. DOI 10.1007/s11056-014-9429-5
de Souza, J. C. A. V., Bender, A. G., Reutemann, A. G., Perreta, M. G., Córdoba, M. S., Tivano, J. C., Barroso, D. G., Gariglio, N. F., Mroginski, L. A y Vegetti, A. V. (2021). Influencia del grado de lignificación de los propágulos de jardín y minijardín clonal en el enraizamiento de estacas y miniestacas de Prosopis alba Griseb. FAVE Sección Ciencias Agrarias, 20(1), 287-304. DOI 10.14409/fa.v20i1.10270
Di Rienzo J. A., Guzmán A.W., y Casanoves F. (2002). A multiple comparisons method based on the distribution of the root node distance of a binary tree. Journal of Agricultural, Biological, and Environmental Statistics, 7,1-14.
Di Rienzo, J. A., Casanoves F., Balzarini M. G., Gonzalez L., Tablada M., y Robledo C. W. (2012). InfoStat. Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina. http://www.infostat.com.ar.
Goyal, Y., y Arya, H. C. (1984). Tissue culture of desert trees: I. Clonal multiplication of Prosopis cineraria by bud culture. Journal of Plant Physiology, 115(3), 183-189. https://doi.org/10.1016/S0176-1617(84)80118-2
Green, B., Tabone, T., y Felker, P. (1990). A comparison of amide and ureide nitrogen sources in tissue culture of tree legume Prosopis alba clone B 2 V 50. Plant cell, tissue and organ culture, 21, 83-86.
Hartmann, H.T.; D.E. Kester; y R.L. Geneve. (2011). Plant propagation principles and practices Prentice Hall.
Hazarika, B. N. (2006). Morpho-physiological disorders in in vitro culture of plants. Scientia Horticulturae, 108(2), 105-120. https://doi.org/10.1016/j.scienta.2006.01.038
Hughes, C. E., Ringelberg, J. J., Lewis, G. P., y Catalano, S. A. (2022). Disintegration of the genus Prosopis L. (Leguminosae, Caesalpinioideae, mimosoid clade). PhytoKeys, 205, 147. doi: 10.3897/phytokeys.205.75379
Jordan, M. (1987). In vitro culture of Prosopis species. In Cell and Tissue Culture in Forestry: Case Histories: Gymnosperms, Angiosperms and Palms (pp. 370-384). Dordrecht: Springer Netherlands.
Jordan, M., Pedraza, J., y Goreux, A. (1985). In vitro propagation studies of three Prosopis species (P. alba, P. chilensis and P. tamarugo) through shoot-tip culture. Gartenbauwissenschaft, 50(6), 265-267.
Maynard, B. K., y Bassuk, N. L. (1991). Stock plant etiolation and stem banding effect on the auxin dose-response of rooting in stem cuttings of Carpinus betulus L.‘Fastigiata’. Plant growth regulation, 10, 305-311. https://doi.org/10.1007/BF00024590
Minchala-Patiño, J., Poma-Angamarca, R., Muñóz-Chamba, L., Yaguana-Arévalo, M., González-Zaruma, D., Eras-Guamán, V. H., C. Rojas-Idrogo, G. E. y Delgado-Paredes, G. E. (2014). Propagación in vitro de Prosopis limensis Benth. in Hook. (Fabaceae-Mimosoideae). Quebracho (Santiago del Estero), 22(2), 88-99.
Morales-Domínguez, J. F., de León, D. S. D., Garcidueñas-Piña, C., y Pérez-Molphe-Balch, E. (2019). Germination, in vitro propagation and soil acclimatization of Acacia farnesiana and Prosopis laevigata. South African Journal of Botany, 124, 345-349. https://doi.org/10.1016/j.sajb.2019.05.034
Murashige, T., y Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 15, 473-497.
Nandwani, D., y Ramawat, K. G. (1991). Callus culture and plantlets formation from nodal explants of Prosopis juliflora (Swartz) DC. Indian J Exp Biol, 29(6), 523-527.
Polivanova, O. B., y Bedarev, V. A. (2022). Hyperhydricity in plant tissue culture. Plants, 11(23), 3313. https://doi.org/10.3390/plants11233313
Rivera Curi, J. C., Cabrera Pintado, R. M., y Bulnes Soriano, F. (2020). Micropropagación de Prosopis pallida (Humb &Bonpl. Ex Willd.) Kunth a partir de yemas apicales. Revista Colombiana de Biotecnología, 22(1), 18-26.
Shekhawat, N. S., Rathore, T. S., Singh, R. P., Deora, N. S., y Rao, S. R. (1993). Factors affecting in vitro clonal propagation of Prosopis cineraria. Plant Growth Regulation, 12, 273-280. https://doi.org/10.1007/BF00027208
Tabone, T. J., Felker, P., Bingham, R. L., Reyes, I., y Loughrey, S. (1986). Techniques in the shoot multiplication of the leguminous tree Prosopis alba clone B2V50. Forest ecology and management, 16(1-4), 191-200. https://doi.org/10.1016/0378-1127(86)90019-8
Vega, C., Aguilar, D., Bessega, C., Teich, I., Acosta, M. C., Cosacov, A., Ewens, M., Vilardi, J., A. N. Sérsic y Verga, A. (2021). Genetic Basis for their Domestication and Conservation. Genetic Variation Patterns of “Algarrobos” from the “Great American Chaco” (Prosopis alba, P. nigra, P. hassleri, P. fiebrigii, P. ruscifolia, P. chilensis, and P. flexuosa). En: M. J. Pastorino & P. Marchelli (Eds.). Low Intensity Breeding of Native Forest Trees in Argentina. Springer. pp. 245-269. https://doi.org/10.1007/978-3-030-56462-9_9
Venier, M. P., Cosacov Martinez, A., López Lauenstein, D., Vega, C., y Verga, A. (2013). Impacto del cambio climático sobre la distribución de Prosopis hassleri y P. alba en la región Chaqueña. Producción Forestal, 7, 35-38.
Venier, P., Ferreras, A. E., Verga, A., y Funes, G. (2015). Germination traits of Prosopis alba from different provenances. Seed Science and Technology, 43(3), 548-553. https://doi.org/10.15258/sst.2015.43.3.14
Verdes, P. (2007). Notas: micropropagación de Prosopis caldenia burk.: estado actual y perspectivas. Revista Científica Agropecuaria, 11(1), 45-51.
Verga, A., y López Lauenstein, D. (2021). Genetic Basis for their Domestication and Conservation. Subtropical dry forests: the main forest ecoregion of Argentina. En: M. J. Pastorino & P. Marchelli (Eds.), Low Intensity Breeding of Native Forest Trees in Argentina. Springer. pp. 217-243. https://doi.org/10.1007/978-3-030-56462-9
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